Yazar "Çanakçı, Sabriye" için listeleme
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Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2T
Yanmış, Derya; Karaoğlu, Hakan; Çolak, Dilşat Nigar; Şal, Fulya Ay; Çanakçı, Sabriye; Beldüz, Ali Osman (2014)The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2T has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography ... -
Cloning, expression and biochemical characterization of a beta-carbonic anhydrase from the soil bacterium Enterobacter sp B13
Eminoğlu, Ayşenur; Vullo, Daniela; Aşık, Aycan; Çolak, Dilşat Nigar; Supuran, Claudiu T.; Çanakçı, Sabriye; Belduz, Ali Osman (Taylor & Francis Ltd, 2016)A recombinant carbonic anhydrase (CA, EC 4.2.1.1) from the soil-dwelling bacterium Enterobacter sp. B13 was cloned and purified by Co2+ affinity chromatography. Bioinformatic analysis showed that the new enzyme (denominated ... -
A new DNA polymerase i from Geobacillus caldoxylosilyticus TK4: Cloning, characterization, and mutational analysis of two aromatic residues
Sandallı, Cemal; Singh, Kamalendra; Modak, Mukund J.; Ketkar, Amit; Çanakçı, Sabriye; Demir, İsmail; Belduz, Ali Osman (Springer, 2009)DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular ...