Yazar "Belduz, Ali Osman" için listeleme
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The ATPase activity of the G2alt gene encoding an aluminium tolerance protein from Anoxybacillus gonensis G2
Beriş, Fatih Şaban; De Smet, Lina; Karaoğlu, Hakan; Çanakcı, Sabriye; Van Beeumen, Jozef; Belduz, Ali Osman (Springer, 2011)The G2ALT gene was cloned and sequenced from the thermophilic bacterium Anoxybacillus gonensis G2. The gene is 666 bp long and encodes a protein 221 amino acids in length. The gene was overexpressed in E. coli and purified ... -
Biochemical characterization of a novel glucose isomerase from Anoxybacillus gonensis G2(T) that displays a high level of activity and thermal stability
Karaoğlu, Hakan; Yanmış, Derya; Sal, Fulya Ay; Çelik, Ayhan; Çanakcı, Sabriye; Belduz, Ali Osman (Elsevier Science Bv, 2013)In the continuing search for novel enzymes suitable for the production of high fructose corn syrup (HFCS), a new glucose isomerase (GI) from the thermophile Anoxybacillus gonensis G2(T) is described. the gene encoding this ... -
Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2(T)
Yanmış, Derya; Karaoğlu, Hakan; Çolak, Dilşat Nigar; Sal, Fulya Ay; Çanakcı, Sabriye; Belduz, Ali Osman (Tubitak Scientific & Technical Research Council Turkey, 2014)The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2(T) has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography ... -
Cloning, expression and biochemical characterization of a beta-carbonic anhydrase from the soil bacterium Enterobacter sp B13
Eminoğlu, Ayşenur; Vullo, Daniela; Aşık, Aycan; Çolak, Dilşat Nigar; Supuran, Claudiu T.; Çanakçı, Sabriye; Belduz, Ali Osman (Taylor & Francis Ltd, 2016)A recombinant carbonic anhydrase (CA, EC 4.2.1.1) from the soil-dwelling bacterium Enterobacter sp. B13 was cloned and purified by Co2+ affinity chromatography. Bioinformatic analysis showed that the new enzyme (denominated ... -
Cloning, expression and characterization of highly thermo- and pH-stable maltogenic amylase from a thermophilic bacterium Geobacillus caldoxylosilyticus TK4
Kolcuoğlu, Yakup; Çolak, Ahmet; Faiz, Özlem; Belduz, Ali Osman (Elsevier, 2010)Maltogenic amylases (MAases), a subclass of cyclodextrin (CD)-hydrolyzing enzymes, belong to glycoside hydrolase family 13. A gene corresponding to MA in Geobacillus caldoxylosilyticus TK4 (GcaTK4MA) was cloned into pET28a(+) ... -
Cloning, expression, purification and characterisation of a thermostable chitinase from Bacillus licheniformis A1
Sandallı, Cemal; Kaçağan, Murat; Çanakcı, Sabriye; Belduz, Ali Osman (University of Milan, 2008)The chitinase B gene (ch/B65) of Bacillus licheniformis A1 (BlicA1) isolated from Diyadin hotspring in Turkey was cloned and sequenced. The gene is 1779 bp long and encodes a protein 592 amino acids with a 35-amino acid ... -
Cloning, expression, purification and characterization of fructose-1,6-bisphosphate aldolase from Anoxybacillus gonensis G2
Ertunga, Nagihan Sağlam; Çolak, Ahmet; Belduz, Ali Osman; Çanakcı, Sabriye; Karaoğlu, Hakan; Sandallı, Cemal (2007)The fructose-1,6-bisphosphate aldolase gene from the thermophilic bacterium, Anoxybacillus gonensis G2, was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame coding for a 30.9 kDa protein ... -
Cloning, purification and characterization of a thermostable carboxylesterase from Anoxybacillus sp. PDF1
Ay, Fulya; Karaoğlu, Hakan; İnan, Kadriye; Çanakcı, Sabriye; Belduz, Ali Osman (Elsevier, 2011)The gene encoding a carboxylesterase from Anoxybacillus sp., PDF1, was cloned and sequenced. The recombinant protein was expressed in Escherichia coli BL21, under the control of isopropyl-?-d-thiogalactopyranoside-inducible ... -
Comparison of DIG-11-dUTP utilization by Geobacillus caldoxylosilyticus TK4, Mycobacterium tuberculosis and Escherichia coli DNA polymerases
Sandallı, Cemal; Çanakcı, Sabriye; Demir, İsmail; Modak, Mukund J.; Belduz, Ali Osman (Springer Link, 2010)DNA polymerases are used for many applications and we comparatively investigated DNA synthesis activity of DNA polymerase I enzymes of Geobacilluscaldoxylosilyticus TK4, Escherichia coli and Mycobacterium tuberculosis with ... -
Morphological variation among atlantic horse mackerel, Trachurus trachurus populations from Turkish coastal waters
Bektaş, Yusuf; Belduz, Ali Osman (2009)Morphological variation among Turkish populations of the Atlantic horse mackerel Trachurus trachurus (Carangidae) was examined for 150 specimens at five different sampling localities (Sea of Marmara (Istanbul and Canakkale), ... -
A new DNA polymerase i from Geobacillus caldoxylosilyticus TK4: Cloning, characterization, and mutational analysis of two aromatic residues
Sandallı, Cemal; Singh, Kamalendra; Modak, Mukund J.; Ketkar, Amit; Çanakçı, Sabriye; Demir, İsmail; Belduz, Ali Osman (Springer, 2009)DNA polymerase I gene was cloned and sequenced from the thermophilic bacterium Geobacillus caldoxylosilyticus TK4. The gene is 2,634 bp long and encodes a protein of 878 amino acids in length. The enzyme has a molecular ... -
PCR based idendification and discrimination of Caranx rhonchus (Pisces, Carangidae) based on nuclear and mtDNA sequences
Bektaş, Yusuf; Belduz, Ali Osman (2009)PCR based nuclear and mitochondrial DNA markers are used to clarify cases of misidentification of adult individuals of fish species with morphological similarities. This problem prevails in the case of false scad (Caranx ... -
The phylogenetic position of Turkish populations within the European Bitterling, Rhodeus amarus (Osteichthyes: Cyprinidae)
Bektaş, Yusuf; Belduz, Ali Osman; Turan, Davut (Taylor & Francis Ltd, 2013)The partial sequence of the mitochondrial cytochrome b gene (1047 bp) was analysed in order to clarify the phylogenetic position of Turkish populations within the European Bitterling, Rhodeus amarus. 17 haplotypes were ... -
Purification, characterization and industrial application of the alkaline protease (akap) from Anoxybacillus kestanbolensis K4
Atasoy, Pınar Yeşilgil; Çanakci, Sabriye; Belduz, Ali Osman (Elsevier Science Bv, 2012)Bu çalışmada yeni bir proteolitik enzim izole edilmiş, saflaştırılmış ve termofilik bir bakteri olan Anoxybacillus kestanbolensis K4 ile karakterize edilmiştir . Enzim, amonyum sülfat fraksiyonasyonu, anyon değişim ... -
Use of rpoB sequences and rep-PCR for phylogenetic study of Anoxybacillus species
İnan, Kadriye; Bektaş, Yusuf; Çanakcı, Sabriye; Belduz, Ali Osman (Springer, 2011)This study was conducted to investigate the applicability of rpoB, which encodes the ? subunit of RNA polymerase, to be used as an alternative to 16S rRNA gene sequence similarity analysis in the thermophilic genus ...
