Recep Tayyip Erdoğan Üniversitesi Kurumsal Akademik Arşivi
DSpace@RTEÜ, Recep Tayyip Erdoğan Üniversitesi tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve yayınların etkisini artırmak için telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Serum and cervical flushing fluid eicosapentaenoic acid levels in patients with unexplained infertility versus healthy controls
(Wiley, 2026) Kapucu Atas, Elif; Doğan Polat, Sibel; Yılmaz, Bülent; Şentürk, Şenol; Ayazoğlu, İlknur Merve; Kağıtçı, Mehmet; Mataracı Karakaş, Sibel; Yılmaz, Adnan
To compare the levels of eicosapentaenoic acid (EPA) in venous blood and cervical flushing fluid between patients with unexplained infertility and a control group, marking the first investigation of its kind in the literature. Recep Tayyip Erdogan University Education and Research Hospital-based cross-sectional study. This study was conducted with a total of 66 women (35 with unexplained infertility and 31 healthy controls) between 20 and 45 who attended the outpatient gynecology clinic between January 2023 and January 2024. Samples for EPA were collected in the midluteal phase and stored at −80°C, analyzed using EPA's ELISA kits. Baseline demographic and hormonal parameters were similar between the unexplained infertility and control groups. Serum EPA levels were lower in the unexplained infertility group, but the difference was not statistically significant. In contrast, cervical flushing fluid EPA concentrations were significantly reduced in women with unexplained infertility (p < 0.001). No correlation was observed between serum and cervical EPA levels (Spearman's ρ = 0.13, p = 0.28). In multivariate analysis, unexplained infertility independently predicted lower cervical EPA concentrations, explaining approximately one-third of the total variance (R2 = 34.3%). Cervical flushing fluid EPA levels were significantly lower in the unexplained infertility group compared to the control group. These findings suggest that local anti-inflammatory lipid imbalance in the cervical microenvironment may contribute to fertility impairment. Further studies are needed to clarify the potential role of EPA as a biomarker or therapeutic target in reproductive disorders.
Identification and development of pathogen- and pest-specific defense–resistance-associated ssr marker candidates assisted by machine learning and discovery of putative QTL hotspots in camellia sinensis
(Multidisciplinary Digital Publishing Institute (MDPI), 2026) Eminoğlu, Ayşenur
In this study, a targeted SSR (Simple Sequence Repeat) marker resource was developed based on genes and protein families associated with pathogen- and pest-related defense–resistance mechanisms in Camellia sinensis. Forty-one genes and protein families reported to show upregulation, increased expression, or functional validation under disease and pest stress were selected, and the corresponding 195 loci were mapped onto the Camellia sinensis cv. Shuchazao genome. SSR screening within gene bodies and gene-flanking regions (±5 kb) identified 5197 SSR loci. Putative QTL hotspot regions were defined using locus-based sliding-window analysis, Z-score calculations, and permutation tests, yielding 633 SSRs filtered at the 99% and 95% significance thresholds. Proteome-wide scans based on conserved amino acid motifs identified multiple loci within the WRKY, NAC, LRR, PRX, and CHI families, and Random Forest analysis was used to prioritize SSRs within these families. Finally, 386 SSR primer sets were designed and evaluated by in silico PCR across six tea genomes. Of these, 245 primers produced amplicons in more than one genome, and 124 exhibited polymorphic information content values greater than 0.500. Overall, the developed SSR panels represent a biologically contextualized and experimentally transferable marker resource targeting defense–resistance-associated genic and gene-proximal regions.
Identification of urinary incontinence prevalence, risk factors, awareness and attitude levels in turkish women: single center cross-sectional study
(Wiley, 2026) Köksal, Zeynep Pehlivan; Özdemir, Vacide Aşık
Urinary incontinence (UI) is a prevalent health issue worldwide, with its frequency increasing with age and negatively impacting individuals across various aspects of life. This study aims to determine the prevalence of UI, identify risk factors, and assess awareness and attitudes in women aged 18–65. This research, conducted as a descriptive and cross-sectional case study, was conducted with 294 randomly selected women working in a hospital in a province of Turkey. Data was collected using face-to-face interviews using the Introductory Information Form and the Urinary Incontinence Awareness and Attitude Scale (URINAS). UI was found in 29% of women aged 18–65. Among those with UI, 34% consulted a physician, and 22% received treatment. Factors influencing UI include age, BMI, vaginal delivery, menopause, family history, having constipation, lifting heavy objects, urinary tract infections, and a desire for Kegel exercise training (p < 0.05). Approximately one-third of women aged 18–65 experience UI. However, only one-third of those seek medical advice and one-fifth receive treatment. Factors that affect UI include age, body mass index, vaginal delivery, menopause, urinary tract infections, heavy lifting, and doing kegel exercises. Women generally accepted UI as a health issue, had high health motivation, did not experience significant restrictions, and displayed moderate levels of coping and fear.
Regional differences in microplastic accumulation in the thornback ray (Raja clavata) along the southern Black Sea coast
(Elsevier, 2026) Eryaşar, Ahmet Raif; Mutlu, Tanju; Dağtekin, Murat; Kaygusuz, Özcan; Gedik, Kenan
Microplastic (MP) pollution is an emerging global concern with potential ecological and human health consequences, yet data on its impacts in demersal cartilaginous fishes remain scarce. This study presents the first assessment of MP ingestion in the thornback ray ( Raja clavata ) specifically focusing on the southern Black Sea coast. A total of 68 individuals collected from 23 stations were examined, yielding 196 MP particles. The mean abundance was 2.88 MPs per individual, with significantly higher values in the eastern Black Sea compared to the western region (Mann–Whitney U, p = 0.036). Fibers dominated the morphological categories (>80 %), while PET and PP were the most prevalent polymer types, and black and blue were the dominant colors. The eastern coast exhibited greater polymer and color diversity, which suggests a potential association with heterogeneous land-based inputs. No significant correlations were found between MP abundance and fish length, weight, or sampling depth. These findings highlight spatial heterogeneity in MP contamination, emphasizing areas of accumulation consistent with regional riverine discharges and coastal pressures. The results highlight the potential importance of targeting land-based sources to support conservation efforts in sensitive marine ecosystems.
Identification of the biological properties of three novel lytic bacteriophages capable of infecting Bacillus anthracis: Novel members of the class Caudoviricetes
(Bailliere Tindall Ltd, 2026) Bozdeveci, Arif; Karaoğlu, Şengül Alpay; Akman, Ayhan; Suyabatmaz, Şeyma; Akpınar, Rahşan; Kızıltepe, Şemistan; Altıntaş, Özlem; Şen, Selahattin
Bacillus anthracis spores can remain viable for many years in regions where anthrax cases are reported or where deceased animals are buried. Anthrax occurrences may be more common in areas where livestock farming is a primary source of livelihood. In this study, 43 Bacillus anthracis isolates were used, which were isolated from samples collected in different provinces of Türkiye. Three lytic bacteriophages(BaTR8, BaTR13, and BaTR16) were isolated from environmental soil samples. These three lytic bacteriophages' biological properties (host range, burst size, and phage morphology) were defined, and molecular methods were used to determine their genomic structures. It was determined that three lytic phages were able to infect 84.1 % of the host bacteria and formed clear phage plaques. Transmission electron microscopy (TEM) analysis identified them as siphoviruses. Bacteriophage genome lengths ranged from 35587 to 37369 kb, and the GC content was approximately 35 %. The genome structures of the three phages were analyzed, determining that they contained 58–62 ORFs and did not encode any tRNA genes. Phylogenetic analyses of the bacteriophages were performed based on whole-genome sequences and conserved signature genes (major capsid, portal, and terminase large subunit proteins), and the phages were taxonomically classified within the class Caudoviricetes. The three phages isolated in this study, together with the Athena phage, which infects Bacillus anthracis, were determined to form a new cluster distinct from the Wbetavirus and Hubeivirus genera. Our study presents, for the first time, the results of a detailed evaluation of Bacillus anthracis phages isolated from Türkiye.
