Yazar "Yıldırım, Melike" için listeleme
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Cloning, expression and characterization of alkaline phosphatase from a thermophilic bacterium Geobacillus caldoxylosilyticus TK4
Çöl, Melek; Çolak, Ahmet; Ertunga, Nagihan Sağlam; Yıldırım, Melike; Özel, Arzu (Turkish Biochemistry Society, 2010)Aim: To clone the gene for alkaline phosphatase (AP) from a novel thermophilic Geobacillus caldoxylosilyticus TK4 strain and to express and characterize this enzyme. Methods: The gene was cloned into pET28a(+) vector and ... -
Purification and characterisation of a polyphenol oxidase from Boletus erythropus and investigation of its catalytic efficiency in selected organic solvents
Özel, Arzu; Çolak, Ahmet; Arslan, Oktay; Yıldırım, Melike (Elsevier, 2010)Polyphenol oxidase (PPO) was purified from Boletus erythropus using a Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column. Optimum pH and temperature were found to be 8.0 and 20 °C, respectively, using 4-methylcatechol ...
