dc.contributor.author | Abbas, Saghir | |
dc.contributor.author | Rashid, Faisal | |
dc.contributor.author | Ülker, Emine | |
dc.contributor.author | Zaib, Sumera | |
dc.contributor.author | Ayub, Khurshid | |
dc.contributor.author | Ullah, Sana | |
dc.contributor.author | Nadeem, Muhammad Arif | |
dc.contributor.author | Yousuf, Sammer | |
dc.contributor.author | Ludwig, Ralf | |
dc.contributor.author | Ali, Saqid | |
dc.contributor.author | Iqbal, Jamshed | |
dc.date.accessioned | 2020-12-19T19:34:52Z | |
dc.date.available | 2020-12-19T19:34:52Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Abbas, S., Rashid, F., Ulker, E., Zaib, S., Ayub, K., Ullah, S., Nadeem, M. A., Yousuf, S., Ludwig, R., Ali, S., & Iqbal, J. (2020). Anticancer evaluation of a manganese complex on HeLa and MCF-7 cancer cells: design, deterministic solvothermal synthesis approach, Hirshfeld analysis, DNA binding, intracellular reactive oxygen species production, electrochemical characterization and density functional theory. Journal of biomolecular structure & dynamics, 39(3), 1068–1081. https://doi.org/10.1080/07391102.2020.1726818 | en_US |
dc.identifier.issn | 0739-1102 | |
dc.identifier.issn | 1538-0254 | |
dc.identifier.uri | https://doi.org/10.1080/07391102.2020.1726818 | |
dc.identifier.uri | https://hdl.handle.net/11436/1191 | |
dc.description | WOS: 000518743700001 | en_US |
dc.description | PubMed: 32041485 | en_US |
dc.description.abstract | Herein, a deterministic solvothermal strategy was employed to synthesize an efficient anticancer agent 'cis-dichlorobis(1,10-phenanthroline)manganese(II)' (Mn(phen)(2)Cl-2). A single-crystal X-ray diffraction analysis revealed that Mn(phen)(2)Cl-2 crystallizes in a triclinic system with the space group P-1. Cyclic voltammetric studies of Mn(phen)(2)Cl-2 indicated that the electrode process occurs only due to complex formation and has a diffusion-controlled mechanism. Density functional theory estimations showed that the Mn(phen)(2)Cl-2 is quite stable and exists in sextet spin state (five unpaired electrons) as the most stable form and hence, Mn(phen)(2)Cl-2 is a high spin complex. Mn(phen)(2)Cl-2 demonstrated significant anticancer potential against HeLa and MCF-7 cancer cells and less toxic behaviour towards normal BHK-21 cells. Fluorescence imaging confirmed that the production of reactive oxygen species (ROS) in HeLa cells by Mn(phen)(2)Cl-2 induces oxidized fluorescence of dichlorofluorescein which emitted fluorescence at 530 nm after excitation at 488 nm. the microscopic investigation of apoptotic effect of Mn(phen)(2)Cl-2 using propidium iodide and 4 ',6-diamidino-2-phenylindole staining indicated that nuclear condensation, cell detachment and shrinkage occur after treatment with IC50 values of Mn(phen)(2)Cl-2. Furthermore, an assessment of caspase-9 and caspase-3 activity after exposure to Mn(phen)(2)Cl-2 in HeLa cells indicated that at IC50 values of Mn(phen)(2)Cl-2, 1.5 fold and 4.8 fold increase in caspase-9 and caspase-3 activity, respectively, occurs. the measurement of mitochondrial membrane potential of a cationic dye (JC-1) showed a decrease in mitochondrial membrane potential in both HeLa and MCF-7 cells depicting that compound might have adopted intrinsic pathway of apoptosis. Ability of Mn(phen)(2)Cl-2 to interact with HS-DNA demonstrates hyperchromicity with slight blue shift from 269 nm to 265 nm showing a non-covalent interaction with Gibbs free energy of Delta G = -14.62 kJ/mol. Communicated by Ramaswamy H. Sarma | en_US |
dc.description.sponsorship | Higher Education Commission of PakistanHigher Education Commission of Pakistan [112-31112-2PS1-514, Ph-V-MG-3/Peridot/RD/HEC/2019] | en_US |
dc.description.sponsorship | Saghir Abbas is grateful to the Higher Education Commission of Pakistan for the financial support to complete this work (PIN. NO. 112-31112-2PS1-514). Jamshed Iqbal is thankful to the Higher Education Commission of Pakistan for providing the financial support through Project No. Ph-V-MG-3/Peridot/R&D/HEC/2019. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Taylor & Francis Inc | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Mn complex | en_US |
dc.subject | Hirshfeld surface analysis | en_US |
dc.subject | Anticancer potential | en_US |
dc.subject | DNA binding | en_US |
dc.subject | Electrochemistry | en_US |
dc.subject | DFT studies | en_US |
dc.title | Anticancer evaluation of a manganese complex on HeLa and MCF-7 cancer cells: design, deterministic solvothermal synthesis approach, Hirshfeld analysis, DNA binding, intracellular reactive oxygen species production, electrochemical characterization and density functional theory | en_US |
dc.type | article | en_US |
dc.contributor.department | RTEÜ, Fen - Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.contributor.institutionauthor | Ülker, Emine | |
dc.identifier.doi | 10.1080/07391102.2020.1726818 | |
dc.identifier.volume | 39 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 1068 | en_US |
dc.identifier.endpage | 1081 | en_US |
dc.relation.journal | Journal of Biomolecular Structure & Dynamics | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |