dc.contributor.author | Houchi, Selma | |
dc.contributor.author | Mahdadi, Rachid | |
dc.contributor.author | Khenchouche, Abdelhalim | |
dc.contributor.author | Song, Junke | |
dc.contributor.author | Zhang, Wen | |
dc.contributor.author | Pang, Xiaocong | |
dc.contributor.author | Zhang, Li | |
dc.contributor.author | Sandallı, Cemal | |
dc.contributor.author | Du, Guanhua | |
dc.contributor.author | Sandallı, Cemal | |
dc.date.accessioned | 2020-12-19T19:40:53Z | |
dc.date.available | 2020-12-19T19:40:53Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Houchi, S., Mahdadi, R., Khenchouche, A., Song, J., Zhang, W., Pang, X., Zhang, Li., Sandallı, C. & Du, G. (2019). Investigation of common chemical components and inhibitory effect on GES-type beta-lactamase (GES22) in methanolic extracts of Algerian seaweeds. Microbial Pathogenesis, 126, 56-62. https://doi.org/10.1016/j.micpath.2018.10.034 | en_US |
dc.identifier.issn | 0882-4010 | |
dc.identifier.uri | https://doi.org/10.1016/j.micpath.2018.10.034 | |
dc.identifier.uri | https://hdl.handle.net/11436/1677 | |
dc.description | HOUCHI, Selma/0000-0002-6922-709X; SANDALLI, Cemal/0000-0002-1298-3687 | en_US |
dc.description | WOS: 000458468100008 | en_US |
dc.description | PubMed: 30393116 | en_US |
dc.description.abstract | This study aimed to investigate the total phenolic content (TPC), the identification of the common compounds by HPLC-ESI-MS and HPLC-ESI-MS-TOF and the inhibitory effects against class A-type beta-lactamase (GES-22 variant, produced recombinantly) in methanolic extracts (MEs) of four Algerian seaweeds [Ulva intestinalis, Codium tomentosum, Dictyota dichotoma and Halopteris scoparia]. the TPC varied among the four species, ranging between 0.93 +/- 0.65 and 2.66 +/- 1.33 mg GAEs/g DW. C.tomentosum had higher total phenol content than other seaweeds while, all of them inhibited uncompetitively GES-22 activity in a dose-dependent manner. Nitrocefin was used as chromogenic substrate to evaluate the inhibitory effect on GES-22. the methanolic extract of D.dichotoma exhibited significant inhibitory effect on GES-22 (IC50 = 13.01 +/- 0.046 mu g/mL) more than clavulanate, sulbactam and tazobactam (classical beta-lactam inhibitors) (IC50 = 68.38 +/- 0.17 mu g/mL, 52.68 +/- 0.64 mu g/mL, and 29.94 +/- 0.01 mu g/mL, respectively). IC50 of the other ME of U.intestinalis, C.tomentostum, and H.scoparia were 16.87 +/- 0.10 mu g/mL, 16.54 +/- 0.048 mu g/mL, and 25.72 +/- 0.15 mu g/mL, respectively. Except H. scoparia, other three seaweed extracts showed almost two times or more inhibition on GES-22. Furthermore, four common compounds in these MEs were identified, alpha-linolenic acid (C18:3 omega 3), linoleic acid (C18:2 omega 6), oleic acid (C18:1 omega 9), the eicosanoid precursors "arachidonic acid" (C20:4 omega 6). Baicalein (C15H10O5) was identified in U.intestinalis and D.dichotoma seaweeds. the fact that all seaweed extracts inhibited the GES-22 better than commercial samples makes these seaweeds candidate for discovering new inhibitors against beta-lactamases. Besides that, they contain important components with potential health benefits. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Academic Press Ltd- Elsevier Science Ltd | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Algerian seaweeds | en_US |
dc.subject | Methanolic extract | en_US |
dc.subject | Phenolic content | en_US |
dc.subject | beta-lactamase (GES-22) | en_US |
dc.subject | Inhibition | en_US |
dc.title | Investigation of common chemical components and inhibitory effect on GES-type beta-lactamase (GES22) in methanolic extracts of Algerian seaweeds | en_US |
dc.type | article | en_US |
dc.contributor.department | RTEÜ, Fen - Edebiyat Fakültesi, Biyoloji Bölümü | en_US |
dc.identifier.doi | 10.1016/j.micpath.2018.10.034 | |
dc.identifier.volume | 126 | en_US |
dc.identifier.startpage | 56 | en_US |
dc.identifier.endpage | 62 | en_US |
dc.relation.journal | Microbial Pathogenesis | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |