dc.contributor.author | Faiz, Özlem | |
dc.contributor.author | Baltaş, Nimet | |
dc.date.accessioned | 2020-12-19T19:49:28Z | |
dc.date.available | 2020-12-19T19:49:28Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Faiz, Ö. & Baltaş, N. (2017). Polyphenol oxidase properties, anti-urease, and anti-acetylcholinesterase activity of Diospyros lotus L. (Plum Persimmon). International Journal of Food Properties, 20(5), 1186-1196. https://doi.org/10.1080/10942912.2016.1205086 | en_US |
dc.identifier.issn | 1094-2912 | |
dc.identifier.issn | 1532-2386 | |
dc.identifier.uri | https://doi.org/10.1080/10942912.2016.1205086 | |
dc.identifier.uri | https://hdl.handle.net/11436/2288 | |
dc.description | WOS: 000399642900020 | en_US |
dc.description.abstract | Diospyros lotus fruit polyphenol oxidase was purified using affinity chromatography, resulting in a 15-fold enrichment in specific activity. the purified enzyme, having 16.5 kDa molecular weight on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, exhibited the highest activity toward 4-methylcatechol. Maximum diphenolase activity was reached at pH 7.0 and 60 degrees C in the presence of 4-methylcatechol. K-m and V-max values were calculated as 3.8 mM and 1250 U/mg protein, respectively. Ascorbic acid was a promising inhibitor with an IC50 value of 0.121 mu M. the activity of the purified enzyme was stimulated by Fe2+, Sr2+, Zn2+, and K+ and deeply inhibited by Hg2+, at 1 mM final concentration. Aqueous extract of Diospyros lotus L. fruit showed strong substantial urease and acetylcholinesterase inhibition, with IC50 values of 1.55 +/- 0.05 and 16.75 +/- 0.11 mg/mL, respectively. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Taylor & Francis Inc | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Plum persimmon | en_US |
dc.subject | Affinity column | en_US |
dc.subject | Enzymatic browning | en_US |
dc.subject | AChE inhibition | en_US |
dc.subject | Urease inhibition | en_US |
dc.title | Polyphenol oxidase properties, anti-urease, and anti-acetylcholinesterase activity of Diospyros lotus L. (Plum Persimmon) | en_US |
dc.type | article | en_US |
dc.contributor.department | RTEÜ, Fen - Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.contributor.institutionauthor | Faiz, Özlem | |
dc.contributor.institutionauthor | Baltaş, Nimet | |
dc.identifier.doi | 10.1080/10942912.2016.1205086 | |
dc.identifier.volume | 20 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.startpage | 1186 | en_US |
dc.identifier.endpage | 1196 | en_US |
dc.ri.edit | oa | en_US |
dc.relation.journal | International Journal of Food Properties | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |