Genetic diversity among selected Japanese quail (Coturnix coturnix japonica) lines using RAPD markers

Abstract

The intra and inter genetic polymorphisms and genetic relatedness of 12 generations selected 3 lines (HBW1, LBW, L); 7 generations selected 1 line (HBW2) and a control (C) line in Japanese quail were investigated by RAPD-PCR. Fourteen DNA samples (7 ?-7 ?) from each line were analyzed using 24 primers. One hundred ninety six polymorphic loci were amplified (150 to 2600 bp) and polymorphism rate was calculated as 99.49 %. Genotypic polymorphism rates were calculated as 63.45%, 31.47%, 42.13%, 35.53% and 36.55% in C., LBW, HBW1, HBW2 and L, respectively. Average heterozygosis within lines (H S), total heterozygosis (R T) and average Shannon index (I) values were 0.169, 0.354 and 0.528, respectively. The genetic variation was highest in C and lowest in LBW line. The genetic identity within line varied from 0.747 (C) to 0.875 (DCA) and between lines varied from 0.603 (C-L) to 0.813 (HBW1-LBW). It was found that genetically similar selection line to base ancestor (C) is HBW2 line (0.667) while farthest is L line (0.494). Thus genetic variation within line and the genetic relationships among lines could be estimated successfully using RAPD-PCR in selected Japanese quail lines. © GSP, India.