Determination of DNA fingerprinting of turkish fat-tailed sheep breeds by RAPD-PCR method

Abstract

The aim of this research was to determine the genetic structure of Turkish fat-tailed sheep breeds (Akkaraman, Güney Karamani{dotless}, Morkaraman, Dağli{dotless}ç, İvesi, Karakaş, Tuj and Norduz) using RAPD (Random Amplified Polymorphic DNA) markers. By using 12 RAPD primers in 125 sheep obtained 218 polymorphic loci which were determined. Average allele number (na), average effective number of allel (ne), average heterozygosity (H), average expected heterozygosity (hj) Shannon's constant (H0), polymorphic loci number (np) and polymorphic loci ratio (Ppoly) were found as 2.00, 1.6256, 0.3636, 0.1784, 0.5408, 218 and 100%, respectively. Used to determine genetic differences between sheep population; genetic diversity coefficient (HT), average heterozygosity within populations (HS) and total heterozygosity were found as 0.5117, 0.1784 and 0.3654, respectively. Genetic distance between sheep populations(D) was changed from 0.1349 to 0.5563. According to cluster analysis result, Akkaraman, Güney Karaman and Morkaraman created a cluster, Tuj breed was more close than İvesi to first cluster. Dağli{dotless}ç and Karakaş took place another cluster. Norduz breed appeared to be most distance from other sheep breeds. © Ankara Üniversitesi Ziraat Fakültesi.