dc.contributor.author | Öztürk, Nevra | |
dc.contributor.author | Tabak, Ahmet | |
dc.contributor.author | Akgöl, Sinan | |
dc.contributor.author | Denizli, Adile | |
dc.date.accessioned | 2020-12-19T20:12:43Z | |
dc.date.available | 2020-12-19T20:12:43Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Öztürk, N., Tabak, A., Akgöl, S. & Denizli, A. (2008). Reversible immobilization of catalase by using a novel bentonite-cysteine (Bent-Cys) microcomposite affinity sorbents. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 322(1-3), 148-154. https://doi.org/10.1016/j.colsurfa.2008.03.001 | en_US |
dc.identifier.issn | 0927-7757 | |
dc.identifier.uri | https://doi.org/10.1016/j.colsurfa.2008.03.001 | |
dc.identifier.uri | https://hdl.handle.net/11436/3926 | |
dc.description.abstract | This study focused on developing bentonite-cysteine (Bent-Cys) microcomposite affinity sorbents (38-105 ?m) for catalase adsorption from aqueous solutions. Pseudo-biospecific affinity ligand l-cysteine was covalently binded onto the bentonite structures. X-ray diffraction and FTIR analysis of Bent-Cys composite affinity sorbents were performed. According to X-Ray diffraction data, cysteine molecule is parallel to the tetrahedral-octahedral-tetrahedral layer (TOT silicate layer) and is a monolayer in the inner layer space of the structure. The surface areas of the Bentonite and Bent-Cys microcomposite structures were found as 33.00 ± 0.30 and 22.80 ± 0.30, respectively. An elemental analysis of immobilized l-cysteine for nitrogen was estimated to be 541.3 ?mol g-1 bentonite. Catalase adsorption onto the microcomposite affinity sorbents from aqueous solutions containing different amounts of catalase at different pH was investigated in a batch system. The maximum catalase adsorption capacity of the Bent-Cys microcomposite affinity sorbents was 175 mg g-1. The non-specific catalase adsorption onto the bentonite was very low (about 2.7 mg g-1). The activity yield decreased with the increase of the enzyme loading. It was observed that there was a significant change between Vmax value of the free catalase and Vmax value of the adsorbed catalase on the Bent-Cys microcomposite affinity sorbents. The Km value of the immobilized enzyme was higher 1.1 times than that of the free enzyme. Optimum operational temperature was 10 °C higher than that of the free enzyme and was significantly broader. It was observed that enzyme could be repeatedly adsorbed and desorbed without loss of adsorption capacity or enzyme activity. © 2008 Elsevier B.V. All rights reserved. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Affinity chromatography | en_US |
dc.subject | Bentonite | en_US |
dc.subject | Catalase | en_US |
dc.subject | Composite systems | en_US |
dc.subject | Cysteine ligand | en_US |
dc.title | Reversible immobilization of catalase by using a novel bentonite-cysteine (Bent-Cys) microcomposite affinity sorbents | en_US |
dc.type | article | en_US |
dc.contributor.department | RTEÜ, Fen - Edebiyat Fakültesi, Kimya Bölümü | en_US |
dc.contributor.institutionauthor | Tabak, Ahmet | |
dc.identifier.doi | 10.1016/j.colsurfa.2008.03.001 | |
dc.identifier.volume | 322 | en_US |
dc.identifier.issue | 01.Mar | en_US |
dc.identifier.startpage | 148 | en_US |
dc.identifier.endpage | 154 | en_US |
dc.relation.journal | Colloids and Surfaces A: Physicochemical and Engineering Aspects | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |