Investigation of heavy metals in tissues and habitats of three edible frogs from Turkiye

Abstract

The soil, water, and organisms have been contaminated by heavy metals due to human activities and industrialization, which has produced a major environmental problem that has a deleterious effect on human health and food quality. Frogs, one of the good bioindicators for environmental pollution, are also among the alternative essential protein sources for humans. In Turkiye, three of these frogs are edible: Pelophylax ridibundus, Pelophylax bedriagae, and Pelophylax caralitanus, also known as Anatolian water frogs. Hence, to assess the possible health risks that might result from consuming frog legs in addition aquatic habitat of Anatolian water frogs, the water, sediment, and frog tissue samples (muscle and liver) were obtained from 11 different provinces covering all regions of Turkiye and analyzed to determine Cd, Cu, Cr, Zn, Pb, and As concentrations. The results revealed considerable variations in heavy metal concentrations among frog tissues, influenced by the sampling sites and species (ANOVA: p < 0.05). The Estimated Daily Intake (EDI) values, calculated based on the average serving size, were also lower than the Provisional Tolerable Daily Intake (PTDI) levels for adult consumers. Furthermore, the study computed the Target Hazard Quotient (THQ) values for heavy metals, all of which were below the critical value of 1, indicating that consuming the hind leg muscles from these frog species would not pose an adverse health risk for humans.