Exogenous application of jasmonates and brassinosteroids alleviates lead toxicity in bamboo by altering biochemical and physiological attributes

Abstract

Exogenous application of phytohormones is getting promising results in alleviating abiotic stresses, particularly heavy metal (HMs). Jasmonate (JA) and brassinosteroid (BR) have crosstalk in bamboo plants, reflecting a burgeoning area of investigation. Lead (Pb) is the most common pollutant in the environment, adversely affecting plants and human health. The current study focused on the foliar application of 10 µM JA and 10 µM BR in both single and combination forms on bamboo plants grown under Pb stress (0, 50, 100, 150 µM) with a completely randomized design by four replications. The study found that applying 10 µM JA and 10 µM BR significantly improves growth and tolerance by reducing oxidative stress, reactive oxygen species including hydrogen peroxide (H2O2, 32.91%), superoxide radicals (O2−•, 33.9%), methylglyoxal (MG, 19%), membrane lipoperoxidation (25.66%), and electrolyte leakage (41.5%) while increasing antioxidant (SOD (18%), POD (13%), CAT (20%), APX (12%), and GR (19%)), non-antioxidant (total phenolics (7%), flavonols (12.3%), and tocopherols (13.8%)), and glyoxylate activity (GLyI (13%), GLyII (19%)), proline content (19%), plant metal chelating capacity (17.3%), photosynthetic pigments (16%), plant growth (10%), and biomass (12%). We found that JA and BR, in concert, boost bamboo species’ Pb tolerance by enhancing antioxidant and glyoxalase cycles, ion chelation, and reducing metal translocation and accumulation. This conclusively demonstrates that utilizing a BR–JA combination form at 10 µM dose may have the potential to yield optimal efficiency in mitigating oxidative stress in bamboo plants. Graphical Abstract: [Figure not available: see fulltext.