dc.contributor.author | Barut, Burak | |
dc.contributor.author | Seyhan, Gökçe | |
dc.contributor.author | Keleş, Turgut | |
dc.contributor.author | Kulein, Barış | |
dc.contributor.author | Bıyıklıoğlu, Zekeriya | |
dc.date.accessioned | 2024-03-28T08:21:13Z | |
dc.date.available | 2024-03-28T08:21:13Z | |
dc.date.issued | 2024 | en_US |
dc.identifier.citation | Barut, B., Seyhan, G., Keleş, T., Kulein, B. & Bıyıklıoğlu, Z. (2024). Nonperipherally and peripherally substituted water-soluble magnesium (II) phthalocyanines and their DNA binding, nuclease activities. Applied Organometallic Chemistry. https://doi.org/10.1002/aoc.7421 | en_US |
dc.identifier.issn | 0268-2605 | |
dc.identifier.uri | https://doi.org/10.1002/aoc.7421 | |
dc.identifier.uri | https://hdl.handle.net/11436/8888 | |
dc.description.abstract | In this study, magnesium (II) phthalocyanines (2a, 3a) and their water-soluble derivatives (2b, 3b) were synthesized via multistep reactions. The structures of these phthalocyanines were identified by FT-IR, NMR, MALDI-TOF, and UV–Vis spectroscopy. The ct-DNA binding (UV–Vis absorption, competitive EB binding, and agarose electrophoresis studies) and supercoiled plasmid DNA nuclease properties (hydrolytic, photonuclease, oxidative nuclease, and photooxidative nuclease) of the water-soluble compounds were investigated using different methods. The DNA binding constant (Kb) values of 2b and 3b were calculated as 8.45 ± (0.25) × 104 and 7.71 ± (0.13) × 104 M−1 at 25°C, respectively. The results showed that 2b had a stronger ct-DNA binding effect than 3b according to Kb and r values. The DNA nuclease studies claimed that both compounds indicated photonuclease activity on plasmid DNA depending on the light dose. Additionally, 2b had a higher photonuclease capacity than 3b. All of these results showed that 2b had promising potential as a photosensitizer agent for photodynamic therapy. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Magnesium | en_US |
dc.subject | Photonuclease | en_US |
dc.subject | Phthalocyanine | en_US |
dc.subject | Plasmid DNA | en_US |
dc.subject | Synthesis | en_US |
dc.title | Nonperipherally and peripherally substituted water-soluble magnesium (II) phthalocyanines and their DNA binding, nuclease activities | en_US |
dc.type | article | en_US |
dc.contributor.department | RTEÜ | en_US |
dc.contributor.institutionauthor | Keleş, Turgut | |
dc.identifier.doi | 10.1002/aoc.7421 | en_US |
dc.relation.journal | Applied Organometallic Chemistry | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |