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Cloning, expression and characterization of alkaline phosphatase from a thermophilic bacterium Geobacillus caldoxylosilyticus TK4
(Turkish Biochemistry Society, 2010)
Aim: To clone the gene for alkaline phosphatase (AP) from a novel thermophilic Geobacillus caldoxylosilyticus TK4 strain and to express and characterize this enzyme. Methods: The gene was cloned into pET28a(+) vector and ...