Histological investigation of the effects of tenoxicam on pulmonary complications of pneumoperitoneum - Tenoxicam reduces lung injuries caused by pneumoperitoneum

Abstract

Objectives. Pneumoperitoneum increases intra-abdominal pressure and generates oxidative stress, which mediates tissue injury. One of the causes of oxidative stress production is an inflammatory reaction. Taking this into consideration, the current animal study was designed, using tenoxicam before a laparoscopy procedure in order to ascertain whether tenoxicam can prevent lung injury caused by pneumoperitoenum. Material and Methods. Fourteen female Wistar rats were randomly divided into two groups: the tenoxicam group (seven rats) and the control group (seven rats). The tenoxicam group was given two doses (totalling 0.5 mg/kg) of intraperitoneal tenoxicam, and the control group was given 0.5 cc of 0.9% NaCl, 12 hours and 1/2 hour before the operation. Under intra-peritoneal anesthesia, a Veress needle was placed in the peritoneal cavity and a 15 mm-Hg pneumoperitoneum was established and maintained for 20 minutes; the peritoneal gas was then desufflated. The lungs were resected at the 180 th minute from the beginning of the operation and were evaluated histopathologically. Histopathological evaluations including intra-alveolar hemorrhage, alveolar edema, congestion and leukocyte infiltration were carried out for both groups. Results. A statistical comparison of the evaluation scores revealed significant differences between the two groups for intra-alveolar hemorrhage (p = 0.007), alveolar edema (p = 0.023) and congestion (p = 0.005) and a non-significant difference for leukocyte infiltration (p = 0.114). Conclusions. Pneumoperitoneum causes injuries to lung tissue; tenoxicam reduces that damage and protects the lungs by decreasing intra-alveolar hemorrhage, alveolar edema and congestion. © Copyright by Wroclaw Medical University.