dc.contributor.author | Duman, Muhammed | |
dc.contributor.author | Satıcıoğlu, İzzet Burçin | |
dc.contributor.author | Büyükekiz, Ayşe Gül | |
dc.contributor.author | Balta, Fikri | |
dc.contributor.author | Altun, Soner | |
dc.date.accessioned | 2020-12-19T19:48:22Z | |
dc.date.available | 2020-12-19T19:48:22Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Duman, M., Saticioglu, I. B., Buyukekiz, A. G., Balta, F., & Altun, S. (2017). Molecular characterization and antimicrobial resistance profile of atypical Citrobacter gillenii and Citrobacter sp. isolated from diseased rainbow trout (Oncorhynchus mykiss). Journal of global antimicrobial resistance, 10, 136–142. https://doi.org/10.1016/j.jgar.2017.05.014 | en_US |
dc.identifier.issn | 2213-7165 | |
dc.identifier.issn | 2213-7173 | |
dc.identifier.uri | https://doi.org/10.1016/j.jgar.2017.05.014 | |
dc.identifier.uri | https://hdl.handle.net/11436/2059 | |
dc.description | saticioglu, Izzet Burcin/0000-0002-2721-3204; Duman, Muhammed/0000-0001-7707-2705 | en_US |
dc.description | WOS: 000410653300028 | en_US |
dc.description | PubMed: 28732787 | en_US |
dc.description.abstract | Aim: Over the last decade, Citrobacter species have been responsible for infections in fish and many species and also new Citrobacter species have been identified. in this study, molecular identifications and the phenotypic and genotypic antimicrobial-resistance characteristics of atypical and typical Citrobacter species were determined. Materials and methods: Seven Citrobacter isolates were investigated from rainbow trout of different lengths with signs of disease. Biochemical characteristics were determined using conventional tests and rapid test kits; moreover, molecular identifications were conducted with 16S rRNA and the gyrB housekeeping gene region. the sequencing results obtained from the gyrB gene region were deposited in the GenBank database and compared with isolates from different countries that were registered in the database. Resistances to florfenicol, sulfonamides, and tetracycline antimicrobials were determined using the broth micro dilution method, and molecular resistance genes against these antimicrobials were identified. All detected resistance genes were confirmed by sequence analyses. Results: It was determined that three of the Citrobacter species with biochemical characteristics were atypical and showed oxidase-positive reactions. All the Citrobacter species were identified as Citrobacter sp. using the 16S rRNA gene; three isolates were identified as Citrobacter gillenii and four as Citrobacter sp. based on gyrB gene sequence analysis. Some isolates were found in the same group as other countries' isolates in the GenBank database, while isolates with high identities were found in different genogroups. All isolates were found to be phenotypically resistant to sulfamethoxazole and susceptible to tetracycline; these isolates' resistance genes included sulI, tetA, tetB, and tetD. (C) 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | Scientific Researcher Department through KUAP(V) project [2014/7]; General Directorate of Agricultural Research and PoliciesGida Tarim ve Hayvancilik Bakanligi [TAGEM/14/AR-GE/26] | en_US |
dc.description.sponsorship | This research was supported by the Scientific Researcher Department through the KUAP(V)-2014/7 project, and the General Directorate of Agricultural Research and Policies with the TAGEM/14/AR-GE/26 project. This research was approved by the Local Ethics Commission (report 2012-14/04). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Sci Ltd | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Citrobacter gillenii | en_US |
dc.subject | Atypical Citrobacter | en_US |
dc.subject | Molecular characterization | en_US |
dc.subject | Antimicrobial resistance genes | en_US |
dc.title | Molecular characterization and antimicrobial resistance profile of atypical Citrobacter gillenii and Citrobacter sp isolated from diseased rainbow trout (Oncorhynchus mykiss) | en_US |
dc.type | article | en_US |
dc.contributor.department | RTEÜ, Su Ürünleri Fakültesi, Su Ürünleri Yetiştiriciliği Bölümü | en_US |
dc.contributor.institutionauthor | Balta, Fikri | |
dc.identifier.doi | 10.1016/j.jgar.2017.05.014 | |
dc.identifier.volume | 10 | en_US |
dc.identifier.startpage | 136 | en_US |
dc.identifier.endpage | 142 | en_US |
dc.relation.journal | Journal of Global Antimicrobial Resistance | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |