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dc.contributor.authorDizman, Yeşim Aktürk
dc.contributor.authorMuratoğlu, Hacer
dc.contributor.authorSandallı, Cemal
dc.contributor.authorNalcacioğlu, Remziye
dc.contributor.authorDemirbağ, Zihni
dc.date.accessioned2020-12-19T19:49:59Z
dc.date.available2020-12-19T19:49:59Z
dc.date.issued2016
dc.identifier.citationDizman, Y. A., Muratoglu, H., Sandalli, C., Nalcacioglu, R., & Demirbag, Z. (2016). Chilo iridescent virus (CIV) ORF 012L encodes a protein with both exonuclease and endonuclease functions. Archives of virology, 161(11), 3029–3037. https://doi.org/10.1007/s00705-016-3007-4en_US
dc.identifier.issn0304-8608
dc.identifier.issn1432-8798
dc.identifier.urihttps://doi.org/10.1007/s00705-016-3007-4
dc.identifier.urihttps://hdl.handle.net/11436/2370
dc.descriptionSANDALLI, Cemal/0000-0002-1298-3687en_US
dc.descriptionWOS: 000385252100008en_US
dc.descriptionPubMed: 27496102en_US
dc.description.abstractChilo iridescent virus (CIV) is the type member of the genus Iridovirus within the family Iridoviridae. the virions of CIV contain a single linear dsDNA molecule that is circularly permuted and terminally redundant. the genome of CIV contains an open reading frame (ORF 012L) encoding a protein homologous to exonuclease II of Schizosaccharomyces pombe. in this study, we focused on the characterization of CIV ORF 012L. the target ORF was cloned into the pET28a vector, expressed in E. coli strain BL21 (DE3) pLysS with an N-terminal His tag and purified to homogeneity by using Ni-NTA affinity chromatography. Biochemical characterization of the purified CIV 012L confirmed that this viral protein is a functional 5'-3' exonuclease that digests 3'-biotin-labelled oligonucleotides and linear double-stranded DNA (dsDNA) molecules from their 5' termini in a highly processive manner. CIV 012L also has a potent endonuclease activity on dsDNA in vitro. in addition, CIV 012L converted supercoiled plasmid DNA (replicative form I, RFI) into the open circular form (RFII) and then open circular form into linear form (RFIII). Endonuclease activity of CIV 012L was optimal in the presence of 10 mM Mg2+ or 30 mM Mn2+ ions and at 150 mM NaCl or KCl salt concentrations. the highest endonuclease activity was obtained at pH 8, and it reached a maximum at 55 A degrees C. the CIV 012L protein showed deficiencies for both double- and single-stranded RNAs.en_US
dc.description.sponsorshipScientific Research Projects Unit of Karadeniz Technical UniversityKaradeniz Technical University [2008.111.004.2]en_US
dc.description.sponsorshipThis study was funded by the Scientific Research Projects Unit of Karadeniz Technical University (Project No.: 2008.111.004.2).en_US
dc.language.isoengen_US
dc.publisherSpringer Wienen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectComplete genome sequenceen_US
dc.subjectBaculovirus alkaline nucleaseen_US
dc.subjectInvertebrate iridovirusen_US
dc.subjectRestriction enzymesen_US
dc.subjectProteomic analysisen_US
dc.titleChilo iridescent virus (CIV) ORF 012L encodes a protein with both exonuclease and endonuclease functionsen_US
dc.typearticleen_US
dc.contributor.departmentRTEÜ, Fen - Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.contributor.institutionauthorDizman, Yeşim Aktürk
dc.contributor.institutionauthorSandallı, Cemal
dc.identifier.doi10.1007/s00705-016-3007-4
dc.identifier.volume161en_US
dc.identifier.issue11en_US
dc.identifier.startpage3029en_US
dc.identifier.endpage3037en_US
dc.relation.journalArchives of Virologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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