• Türkçe
    • English
  • Türkçe 
    • Türkçe
    • English
  • Giriş
Öğe Göster 
  •   RTEÜ
  • Araştırma Çıktıları | TR-Dizin | WoS | Scopus | PubMed
  • WoS İndeksli Yayınlar Koleksiyonu
  • Öğe Göster
  •   RTEÜ
  • Araştırma Çıktıları | TR-Dizin | WoS | Scopus | PubMed
  • WoS İndeksli Yayınlar Koleksiyonu
  • Öğe Göster
JavaScript is disabled for your browser. Some features of this site may not work without it.

Effects of alendronate and pamidronate on apoptosis and cell proliferation in cultured primary human gingival fibroblasts

Thumbnail

Göster/Aç

Full Text / Tam Metin (275.4Kb)

Erişim

info:eu-repo/semantics/closedAccess

Tarih

2015

Yazar

Soydan, Sıdıka Sinem
Araz, Kenan
Şenel, Firdevs
Yurtcu, Erkan
Helvacıoğlu, Fatma
Dağdeviren, Attila
Tekindal, Mustafa Agah
Şahin, Feride İffet

Üst veri

Tüm öğe kaydını göster

Künye

Soydan, S., Araz, K., Senel, F., Yurtcu, E., Helvacioglu, F., Dagdeviren, A., Tekindal, M., & Sahin, F. (2015). Effects of alendronate and pamidronate on apoptosis and cell proliferation in cultured primary human gingival fibroblasts. Human & Experimental Toxicology, 1073–1082. https://doi.org/10.1177/0960327115569808

Özet

Data arising from the recent literature directed the researchers to study on the degree and extent of bisphosphonate toxicity on oral mucosa in further detail. the aim of this study is to determine the half maximal inhibitory concentration of pamidronate (PAM) and alendronate (ALN) on human gingival fibroblasts in vitro using 3-[4.5-thiazol-2-yl]-2.5-diphenyltetrazolium bromide (MTT) assay and to evaluate the effects of both agents on the proliferation and apoptotic indices. Cells used in the study were generated from human gingival specimens and divided into alendronate (n = 240), PAM (n = 240), and control groups (n = 60). Based on the MTT assay results, 10(-4), 10(-5), 10(-6), and 10(-7) M concentrations of both drugs were administered and the effects were evaluated for 6, 12, 24, 48, or 72 h periods. An indirect immunofluorescence technique was used to evaluate apoptotic (anti-caspase 3) and proliferation (anti-Ki67) indices. Toxicity of both PAM and ALN was found to be the most potent at 10(-4)-10(-5) M range. the apoptotic index of PAM group was found to be significantly higher than ALN group for all concentrations especially at 24 h incubation time (p < 0.05). the decrease in the proliferation index was found similar in first 48 h for both drugs; however, after 72 h of incubation decrease in proliferation index in PAM group was found to be significantly higher (p < 0.05). Micromolar concentrations of not only PAM but also ALN rapidly affect cells generated from human oral gingival tissue by inducing apoptosis together with inhibition of proliferation. Cytotoxic effects of both ALN and PAM on primary human gingival fibroblasts, which cause significant changes in apoptotic and proliferative indices as shown in this in vitro study, suggests that the defective epithelialization of oral mucosa is possibly a major factor on the onset of bisphosphonate-related osteonecrosis of the jaw cases.

Kaynak

Human & Experimental Toxicology

Cilt

34

Sayı

11

Bağlantı

https://doi.org/10.1177/0960327115569808
https://hdl.handle.net/11436/2742

Koleksiyonlar

  • DŞHF, Klinik Bilimler Bölümü Koleksiyonu [254]
  • PubMed İndeksli Yayınlar Koleksiyonu [2443]
  • Scopus İndeksli Yayınlar Koleksiyonu [6023]
  • WoS İndeksli Yayınlar Koleksiyonu [5260]



DSpace software copyright © 2002-2015  DuraSpace
İletişim | Geri Bildirim
Theme by 
@mire NV
 

 




| Yönerge | Rehber | İletişim |

DSpace@RTEÜ

by OpenAIRE
Gelişmiş Arama

sherpa/romeo

Göz at

Tüm DSpaceBölümler & KoleksiyonlarTarihe GöreYazara GöreBaşlığa GöreKonuya GöreTüre GöreDile GöreBölüme GöreKategoriye GöreYayıncıya GöreErişim ŞekliKurum Yazarına GöreBu KoleksiyonTarihe GöreYazara GöreBaşlığa GöreKonuya GöreTüre GöreDile GöreBölüme GöreKategoriye GöreYayıncıya GöreErişim ŞekliKurum Yazarına Göre

Hesabım

GirişKayıt

İstatistikler

Google Analitik İstatistiklerini Görüntüle

DSpace software copyright © 2002-2015  DuraSpace
İletişim | Geri Bildirim
Theme by 
@mire NV
 

 


|| Rehber|| Yönerge || Kütüphane || Recep Tayyip Erdoğan Üniversitesi || OAI-PMH ||

Recep Tayyip Erdoğan Üniversitesi, Rize, Türkiye
İçerikte herhangi bir hata görürseniz, lütfen bildiriniz:

Creative Commons License
Recep Tayyip Erdoğan Üniversitesi Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..

DSpace@RTEÜ:


DSpace 6.2

tarafından İdeal DSpace hizmetleri çerçevesinde özelleştirilerek kurulmuştur.