Effect of mirtazapine on gastric oxidative stress and DNA injury created with methotrexate in rats

Abstract

In this study, effect of mirtazapine on gastric oxidative stress and DNA injury created with methotrexate was investigated. Experimental results showed that GSH (nmol/g protein), MDA (?mol/g protein) and MPO (?/g protein) in the gastric tissue of the control group rats receiving methotrexate are 4.97 ± 0.37, 2.78 ± 0.30 and 3.12 ± 0.18, respectively. GSH, MDA and MPO measurements in the gastric tissue of rats receiving mirtazapine + methotrexate were detected to be 9.23 ± 0.51(p < 0.0001), 1.80 ± 0.31(p < 0.0001) and 1.63 ± 0.25 (p < 0.0001), respectively. GSH, MDA and MPO values in the intact rat group were found 8 ± 0.38 (p < 0,0001), 1.63 ± 0.28 (p < 0.0001) and 1.37 ± 0.21 (p < 0.0001), respectively. In addition, while 8-ohdG/dG quantity that DNA injury product in the control group administered methotrexate was 2.4 ± 0.11 pmol/L, this quantity was 1.3 ± 0.14 pmol/L (p < 0.001), 1.1 ± 0.10 pmol/L (p < 0.001) in mirtazapine and intact group, respectively. As a result, it was seen that mirtazapine prevents increase of oxidative stress and DNA injury created with methotreaxete in the gastric tissue of rat.