Melatonin improves periodontitis-induced kidney damages by decreasing inflammatory stress and apoptosis in rats
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Erişim
info:eu-repo/semantics/closedAccessTarih
2020Yazar
Köse, OğuzBayrakdar, Sevda Kurt
Ünver, Büşra
Altın, Ahmet
Akyıldız, Kerimali
Mercantepe, Tolga
Bostan, Semih Alperen
Arabacı, Taner
Türker Şener, Leyla
Köse, Taha Emre
Tümkaya, Levent
Yılmaz, Adnan
Kuluşlu, Göker
Üst veri
Tüm öğe kaydını gösterKünye
Kose, O., Kurt Bayrakdar, S., Unver, B., Altin, A., Akyildiz, K., Mercantepe, T., Bostan, S. A., Arabaci, T., Turker Sener, L., Emre Kose, T., Tumkaya, L., Yilmaz, A., & Kuluslu, G. (2021). Melatonin improves periodontitis-induced kidney damage by decreasing inflammatory stress and apoptosis in rats. Journal of periodontology, 92(6), 22–34. https://doi.org/10.1002/JPER.20-0434Özet
Background: Two main aims of this animal study were to inspect the possible effects of periodontitis on the structure and functions of the kidneys and the therapeutic effectiveness of melatonin. Methods: Twenty-four male Sprague-Dawley rats were randomly divided into three groups: control, experimental periodontitis (Ep), and Ep-melatonin (Ep-Mel). Periodontitis was induced by placing 3.0-silk sutures sub-paramarginally around the cervix of right-left mandibular first molars and maintaining the sutures for 5 weeks. Then melatonin (10 mg/kg body weight/day, 14 days), and the vehicle was administered intraperitonally. Mandibular and kidney tissue samples were obtained following the euthanasia. Periodontal bone loss was measured via histological and microcomputed tomographic slices. On right kidney histopathological and immunohistochemical, and on the left kidney biochemical (malonyl-aldehyde [MDA], glutathione, oxidative stress [OSI], tumor necrosis factor [TNF]-?, interleukin [IL]-1?, matrix metalloproteinase [MMP]-8, MMP-9, and cathepsin D levels) evaluations were performed. Renal functional status was analyzed by levels of serum creatinine, urea, cystatin-C, and urea creatinine. Results: Melatonin significantly restricted ligature-induced periodontal bone loss (P <0.01) and suppressed the levels of proinflammatory cytokines (TNF-? and IL-1?), oxidative stress (MDA and OSI), and proteases (MMP-8, MMP-9, and CtD) that was significantly higher in the kidneys of the rats with periodontitis (P <0.05). In addition, periodontitis-related histological damages and apoptotic activity were also significantly lower in the Ep-Mel group (P <0.05). However, the markers of renal function of the Ep group were detected slightly impaired in comparison with the control group (P >0.05); and the therapeutic activity of melatonin was limited (P >0.05). Conclusion: Melatonin restricts the periodontitis-induced inflammatory stress, apoptosis, and structural but not functional impairments. © 2020 American Academy of Periodontology