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dc.contributor.authorÇelik, Önder
dc.contributor.authorÇelik, Nilüfer
dc.contributor.authorZan, Ertan
dc.contributor.authorDalkılıç, Semih
dc.contributor.authorSağlam, Aylin
dc.contributor.authorYurci, Arzu
dc.contributor.authorŞentürk, Şenol
dc.contributor.authorŞahin, Erdem
dc.contributor.authorŞahin, Mefkure Eraslan
dc.date.accessioned2020-12-19T19:32:19Z
dc.date.available2020-12-19T19:32:19Z
dc.date.issued2020
dc.identifier.citationCelik, O., Celik, N., Zan, E., Dalkilic, S., Saglam, A., Yurci, A., Senturk, S., Sahin, E., & Sahin, M. E. (2020). Genome-wide expression analysis of endometrium before and after endometrioma surgery. European journal of obstetrics, gynecology, and reproductive biology, 253, 141–147. https://doi.org/10.1016/j.ejogrb.2020.08.036en_US
dc.identifier.issn0301-2115
dc.identifier.issn1872-7654
dc.identifier.urihttps://doi.org/10.1016/j.ejogrb.2020.08.036
dc.identifier.urihttps://hdl.handle.net/11436/989
dc.descriptionyurci, arzu/0000-0003-4808-9019; Eraslan Sahin, Mefkure/0000-0001-6484-9132; SAHIN, Erdem/0000-0001-9492-6223en_US
dc.descriptionWOS: 000575090700024en_US
dc.descriptionPubMed: 32866857en_US
dc.description.abstractObjective: This study was planned to investigate possible alteration in the number of differentially expressed genes (DEGs) in eutopic endometrium before and after laparoscopic removal of the ovarian endometrioma. Study design: Six infertile women with ovarian endometrioma who underwent laparoscopic endometrioma cystectomy and six fertile control subjects who underwent tubal sterilization were included the study. Endometrial samples were collected before and 3 months after surgery throughout the mid-luteal phase. Genome-wide expression profiling was performed with Illumina Human HT-12V4 microchip, a high density silica bead-based microarray which utilizing more than 47.000 probs. Illumina microsequence system was used to assess detection of p value for each probe in every sample. Probes revealing significant assessment (p < .05) were selected for comparative analysis. Results: We have detected 1478 DEGs in the comparison between endometrium of women with endometrioma and fertile controls.118 out of 1478 genes (7.9 %) were significantly increased or decreased more than 1.5-fold in their expression. When the preoperative values of the control and patient groups are compared the number of DEGs was 243 (7.5 %). in 9 out of 243 genes, the fold change was found to be 1.5 and more (3.7 %). Comparison of the number of DEGs after endometrioma surgery and tubal ligation revealed that expression patterns of 1036 genes (33.7 %) were changed in endometrioma group. in 105 out of 1036 genes, the fold change was found to be 1.5 and above (10 %). A comparison using 2706 probes revealed changes in the expression patterns of 106 different genes (3.9 %) after endometrioma resection. in 4 out of 106 genes, the fold change was found to be 1.5 and above (3.7 %). the comparison using 6035 probes revealed changes in the expression patterns of 93 genes (1.5 %) after tubal ligation. None of the 93 genes had a fold change of 1.5 or higher. the number of DEGs in endometrioma groups after surgery was approximately 3-fold higher than control group. Conclusions: Endometrium of women with endometrioma displayed abnormal expression of genes associated with implantation, immunological, endocrine and neuracrine functions. Positive alteration of the expression pattern of DEGs and signal transduction pathways following endometrioma surgery can improve the receptive capacity and implantation rates of eutopic endometrium. (C) 2020 Elsevier B.V. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEndometrioma surgeryen_US
dc.subjectEutopic endometriumen_US
dc.subjectReceptivityen_US
dc.subjectGenome-wide expression analysisen_US
dc.subjectIlluminaen_US
dc.titleGenome-wide expression analysis of endometrium before and after endometrioma surgeryen_US
dc.typearticleen_US
dc.contributor.departmentRTEÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.contributor.institutionauthorŞentürk, Şenol
dc.identifier.doi10.1016/j.ejogrb.2020.08.036
dc.identifier.volume253en_US
dc.identifier.startpage141en_US
dc.identifier.endpage147en_US
dc.relation.journalEuropean Journal of Obstetrics & Gynecology and Reproductive Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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