Evaluation of the effects of dexmedetomidine on liver damage secondary to renal ischemia-reperfusion

Abstract

Background: the aim of this study was to evaluate the hepatic protective effects of dexmedetomidine in a rat model of renal ischemia-reperfusion (I/R) injury. Methodology: We selected 18 albino rats and randomly divided them into 3 equal groups (n = 6); control group (Group C), ischemia/reperfusion group (Group I/R), and Group D+I/R, in which dexmedetomidine was given and I/R was administered. the right renal pedicle was ligated to induce I/R in Group-I/R and Group-D+I/R, the left renal pedicle was clamped with an atraumatic vascular clamp to induce ischemia for 120 min and then reperfusion was performed for 120 min. in Group D+I/R, dexmedetomidine 100 mu/kg was administered intraperitoneally 30 min before the administration of before renal I/R. Histopathological changes in liver tissue, caspase-3 activity, glutathione activity, and malondialdehyde level were evaluated through serum renal and liver function tests. Results: After ischemia/reperfusion, the levels of malondialdehyde, glutathione, aspartate aminotransaminase, alanine aminotransaminase, blood urea nitrogen, creatinine, and caspase-3 were increased and these parameters were observed to be improved followed by the administration of dexmedetomidine. After ischemia/reperfusion, histopathological deterioration was also observed, and less histopathological deterioration was observed in rats given dexmedetomidine. Conclusion: the effects of renal ischemia-reperfusion on hepatic tissue were evaluated histopathologically, immunologically, and biochemically. It was observed that renal and liver damage occurred after ischemia-reperfusion and dexmedetomidine reduced damages in both kidney and liver.