dc.contributor.author | Öçiçek, Adalet | |
dc.contributor.author | Çetin, Nihal | |
dc.contributor.author | Çimen, Ferda Keskin | |
dc.contributor.author | Tuümkaya, Levent | |
dc.contributor.author | Malkoç, İsmail | |
dc.contributor.author | Gulaboğlu, Mine | |
dc.contributor.author | Yaralı, Oğuzhan | |
dc.contributor.author | Süleyman, Bahadır | |
dc.date.accessioned | 2020-12-19T19:56:11Z | |
dc.date.available | 2020-12-19T19:56:11Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Ozcicek, A., Cetin, N., Keskin Cimen, F., Tumkaya, L., Malkoc, I., Gulaboglu, M., Yarali, O., & Suleyman, B. (2016). The Impact of Resveratrol on Oxidative Stress Induced by Methotrexate in Rat Ileum Tissue: Evaluation of Biochemical and Histopathological Features and Analysis of Gene Expression. Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 25(2), 181–186. https://doi.org/10.1159/000442020 | en_US |
dc.identifier.issn | 1011-7571 | |
dc.identifier.issn | 1423-0151 | |
dc.identifier.uri | https://doi.org/10.1159/000442020 | |
dc.identifier.uri | https://hdl.handle.net/11436/2686 | |
dc.description | Cetin, Nihal/0000-0003-3233-8009; Malkoc, Ismail/0000-0002-9221-510X | en_US |
dc.description | WOS: 000370081000014 | en_US |
dc.description | PubMed: 26517535 | en_US |
dc.description.abstract | Objective: the aim of this study was to assess the impact of resveratrol (RST) on oxidative stress induced by methotrexate in rat ileum tissue. Materials and Methods: Twenty-four rats were divided into 4 groups with 6 in each group. Each rat was orally administered the following every day for 30 days: group 1 (MTXG), methotrexate (MTX; 5 mg/kg); group 2 (RMTXG), MTX (5 mg/kg) plus RST (25 mg/kg/day); group 3 (RSTG), RST alone (25 mg/kg/day), and group 4 (controls), distilled water. After the rats had been sacrified, the ilea were removed for the assessment of malondialdehyde (MDA), total glutathione (tGSH) and glutathione peroxidase (GSH-Px). Gene expression analyses for interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) and myeloperoxidase (MPO) were also performed. Hematoxylin and eosin-stained paraffinembedded sections of the ileum were analyzed under a light microscope and the findings were recorded. Statistical analyses of the data were performed using one-way ANOVA. Results: the administration of MTX in group 1 yielded a higher level of MDA (8.33 +/- 2.5 mu mol/g protein, p < 0.001) and lower levels of tGSH (0.97 +/- 0.29 nmol/g protein) and GSH-Px (5.22 +/- 0.35 U/g protein, p < 0.001) compared to the other groups. MTX also increased IL-1 beta (40.33 +/- 5.43 gene expression levels), TNF-alpha (6.08 +/- 0.59) and MPO gene expression (9 +/- 1.41) in group 1 compared to the controls (11.33 +/- 2.07, 2.15 +/- 0.33 and 3.43 +/- 0.48, respectively, p < 0.001). the impact of RST on IL-1 beta, TNF-alpha and MPO gene expression induced by MTX was observed as a reversal of these findings (p < 0.05). Severe inflammation, damage to the villus epithelium and crypt necrosis was observed histopathologically in the MTXG group, whereas only mild inflammation was seen in the RMTXG group. Conclusion: in this study, ileal damage caused by MTX was inhibited by RST. (C) 2015 S. Karger AG, Basel | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Karger | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Gene expression | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | Resveratrol | en_US |
dc.subject | Ileum tissue | en_US |
dc.subject | Rat | en_US |
dc.title | The impact of resveratrol on oxidative stress induced by methotrexate in rat ileum tissue: evaluation of biochemical and histopathological features and analysis of gene expression | en_US |
dc.type | article | en_US |
dc.contributor.department | RTEÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü | en_US |
dc.contributor.institutionauthor | Tuümkaya, Levent | |
dc.identifier.doi | 10.1159/000442020 | |
dc.identifier.volume | 25 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.startpage | 181 | en_US |
dc.identifier.endpage | 186 | en_US |
dc.ri.edit | oa | en_US |
dc.relation.journal | Medical Principles and Practice | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |