Basit öğe kaydını göster

dc.contributor.authorÇetiz, Mehmet Veysi
dc.contributor.authorTurumtay, Emine Akyüz
dc.contributor.authorBurnaz, Nesibe Arslan
dc.contributor.authorÖzhatay, Fatma Neriman
dc.contributor.authorKaya, Erdal
dc.contributor.authorMemon, Abdulrezzak
dc.contributor.authorTurumtay, Halbay
dc.date.accessioned2023-09-05T05:45:11Z
dc.date.available2023-09-05T05:45:11Z
dc.date.issued2023en_US
dc.identifier.citationCetiz, M. V., Turumtay, E. A., Burnaz, N. A., Özhatay, F. N., Kaya, E., Memon, A., & Turumtay, H. (2023). Phylogenetic analysis based on the ITS, matK and rbcL DNA barcodes and comparison of chemical contents of twelve Paeonia taxa in Türkiye. Molecular biology reports, 50(6), 5195–5208. https://doi.org/10.1007/s11033-023-08435-zen_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.urihttps://doi.org/10.1007/s11033-023-08435-z
dc.identifier.urihttps://hdl.handle.net/11436/8243
dc.description.abstractBackgroudTwelve taxa of herbaceous Paeonia species were recorded in Turkiye. All definitions were performed morphologically and/or anatomically and there is no study based on DNA barcode sequences. Three barcode regions were sequenced to determine the phylogenetic relationships of Turkish Paeonia taxa. The chemical comparison of roots was also investigated.Methods and resultsThe taxons were collected between May and June 2021 from nine cities. Leaf materials were used for DNA isolation and ITS, matK and rbcL regions were amplified and sequenced. There was no difference among taxa in terms of rbcL sequences. But the ITS and matK regions distinguished 12 taxa and structured them in two groups. ITS region distinguished P. peregrina, P. arietina, and P. tenuifolia from other taxa, while matK region distinguished P. arietina and P. witmanniana from other taxa. Both barcode sequences actually showed that the registration of P. mascula subsp. arasicola was actually 100% similar to P. arietina. ITS was the most polymorphic region (n = 54) followed by matK (n = 9). These sequences could successfully discriminate Paoenia species from each other and diploid P. tenuifolia. The methanolic root (100 gr) extracts were examined for total phenolic and flavonoid content, and antioxidant activities. Significant variation was found for polyphenolic content, and antioxidant properties (TPC from 204.23 to 2343.89 mg, TFC from 7.73 to 66.16 mg, and FRAP from 523.81 to 4338.62 mg). SC50 values of ABTS and DPPH were ranged from 115.08 to 1115.52 mu g/ml and 73.83 to 963.59 mu g/ml, respectively.ConclusionIt was concluded that 11 of 12 taxa had differences in terms of ITS and matK sequences and these region must be used for the correct identification of Turkish Paeonia.en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPaeoniaen_US
dc.subjectITS regionen_US
dc.subjectMatKen_US
dc.subjectMolecular phylogenyen_US
dc.subjectChemical contenten_US
dc.subjectTurkiyeen_US
dc.titlePhylogenetic analysis based on the ITS, matK and rbcL DNA barcodes and comparison of chemical contents of twelve Paeonia taxa in Turkiyeen_US
dc.typearticleen_US
dc.contributor.departmentRTEÜ, Fen - Edebiyat Fakültesi, Kimya Bölümüen_US
dc.contributor.institutionauthorTurumtay, Emine Akyüz
dc.identifier.doi10.1007/s11033-023-08435-zen_US
dc.relation.journalMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Bu öğenin dosyaları:

Thumbnail

Bu öğe aşağıdaki koleksiyon(lar)da görünmektedir.

Basit öğe kaydını göster