Yazar "Çolak, Ahmet" için listeleme
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Cloning, expression and characterization of alkaline phosphatase from a thermophilic bacterium Geobacillus caldoxylosilyticus TK4
Çöl, Melek; Çolak, Ahmet; Ertunga, Nagihan Sağlam; Yıldırım, Melike; Özel, Arzu (Turkish Biochemistry Society, 2010)Aim: To clone the gene for alkaline phosphatase (AP) from a novel thermophilic Geobacillus caldoxylosilyticus TK4 strain and to express and characterize this enzyme. Methods: The gene was cloned into pET28a(+) vector and ... -
Cloning, expression and characterization of highly thermo- and pH-stable maltogenic amylase from a thermophilic bacterium Geobacillus caldoxylosilyticus TK4
Kolcuoğlu, Yakup; Çolak, Ahmet; Faiz, Özlem; Belduz, Ali Osman (Elsevier, 2010)Maltogenic amylases (MAases), a subclass of cyclodextrin (CD)-hydrolyzing enzymes, belong to glycoside hydrolase family 13. A gene corresponding to MA in Geobacillus caldoxylosilyticus TK4 (GcaTK4MA) was cloned into pET28a(+) ... -
Cloning, expression and characterization of xylose isomerase from thermophilic Geobacillus caldoxylosilyticus TK4 strain
Faiz, Özlem; Çolak, Ahmet; Kolcuoğlu, Yakup; Ertunga, Nagihan Sağlam (Turkish Biochemistry Society, 2011)Aim: To clone the gene of Xylose isomerase (XyI) from thermophilic Geobacillus cal-doxylosilyticus TK4 strain and to express and characterize this enzyme. Methods: XyI gene of Geobacillus caldoxylolyticus was cloned into ... -
Cloning, expression, purification and characterization of fructose-1,6-bisphosphate aldolase from Anoxybacillus gonensis G2
Ertunga, Nagihan Sağlam; Çolak, Ahmet; Belduz, Ali Osman; Çanakcı, Sabriye; Karaoğlu, Hakan; Sandallı, Cemal (2007)The fructose-1,6-bisphosphate aldolase gene from the thermophilic bacterium, Anoxybacillus gonensis G2, was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame coding for a 30.9 kDa protein ... -
DNA binding, antioxidant and antimicrobial activities of homo- and heteronuclear copper(II) and nickel(II) complexes with new oxime-type ligands
Çolak, Ahmet; Terzi, Ülkü; Çöl, Melek; Karaoğlu, Şengül Alpay; Karaböcek, Serdar; Küçükdumlu, Aslıgül; Ayaz, Faik Ahmet (Elsevier, 2010)Some homo- and heteronuclear Cu(II) and Ni(II) complexes of new oxime-type ligands were tested against several pathogenic microorganisms to assess their antimicrobial potentials. The antimicrobial activities of complexes ... -
Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil
Bu çalışma, Trichoderma harzianum lipazının karakterizasyonu hakkında ilk rapordur. Yeni bir suş olan Trichoderma harzianum IDM14D topraktan izole edildi. İzole edilen bu suş, lipaz üretimi için çalkalamalı kültürde 30 ... -
Nutritional value of some wild edible mushrooms from Black Sea Region (Turkey)
Ayaz, Faik Ahmet; Torun, Hülya; Özel, Arzu; Çöl, Melek; Duran, Celal; Sesli, Ertuǧrul; Çolak, Ahmet (Turkish Biochemistry Society, 2011)Objectives: The aim of this study is to profile the nutritional content of eight edible mushrooms collected from East Black Sea region in Turkey. Methods: The eight different wild edible mushrooms (Boletopsis leucomelaena, ... -
Purification and characterisation of a polyphenol oxidase from Boletus erythropus and investigation of its catalytic efficiency in selected organic solvents
Özel, Arzu; Çolak, Ahmet; Arslan, Oktay; Yıldırım, Melike (Elsevier, 2010)Polyphenol oxidase (PPO) was purified from Boletus erythropus using a Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column. Optimum pH and temperature were found to be 8.0 and 20 °C, respectively, using 4-methylcatechol ...