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Anticancer evaluation of a manganese complex on HeLa and MCF-7 cancer cells: design, deterministic solvothermal synthesis approach, Hirshfeld analysis, DNA binding, intracellular reactive oxygen species production, electrochemical characterization and density functional theory

Erişim

info:eu-repo/semantics/closedAccess

Tarih

2020

Yazar

Abbas, Saghir
Rashid, Faisal
Ülker, Emine
Zaib, Sumera
Ayub, Khurshid
Ullah, Sana
Nadeem, Muhammad Arif
Yousuf, Sammer
Ludwig, Ralf
Ali, Saqid
Iqbal, Jamshed

Üst veri

Tüm öğe kaydını göster

Künye

Abbas, S., Rashid, F., Ulker, E., Zaib, S., Ayub, K., Ullah, S., Nadeem, M. A., Yousuf, S., Ludwig, R., Ali, S., & Iqbal, J. (2020). Anticancer evaluation of a manganese complex on HeLa and MCF-7 cancer cells: design, deterministic solvothermal synthesis approach, Hirshfeld analysis, DNA binding, intracellular reactive oxygen species production, electrochemical characterization and density functional theory. Journal of biomolecular structure & dynamics, 39(3), 1068–1081. https://doi.org/10.1080/07391102.2020.1726818

Özet

Herein, a deterministic solvothermal strategy was employed to synthesize an efficient anticancer agent 'cis-dichlorobis(1,10-phenanthroline)manganese(II)' (Mn(phen)(2)Cl-2). A single-crystal X-ray diffraction analysis revealed that Mn(phen)(2)Cl-2 crystallizes in a triclinic system with the space group P-1. Cyclic voltammetric studies of Mn(phen)(2)Cl-2 indicated that the electrode process occurs only due to complex formation and has a diffusion-controlled mechanism. Density functional theory estimations showed that the Mn(phen)(2)Cl-2 is quite stable and exists in sextet spin state (five unpaired electrons) as the most stable form and hence, Mn(phen)(2)Cl-2 is a high spin complex. Mn(phen)(2)Cl-2 demonstrated significant anticancer potential against HeLa and MCF-7 cancer cells and less toxic behaviour towards normal BHK-21 cells. Fluorescence imaging confirmed that the production of reactive oxygen species (ROS) in HeLa cells by Mn(phen)(2)Cl-2 induces oxidized fluorescence of dichlorofluorescein which emitted fluorescence at 530 nm after excitation at 488 nm. the microscopic investigation of apoptotic effect of Mn(phen)(2)Cl-2 using propidium iodide and 4 ',6-diamidino-2-phenylindole staining indicated that nuclear condensation, cell detachment and shrinkage occur after treatment with IC50 values of Mn(phen)(2)Cl-2. Furthermore, an assessment of caspase-9 and caspase-3 activity after exposure to Mn(phen)(2)Cl-2 in HeLa cells indicated that at IC50 values of Mn(phen)(2)Cl-2, 1.5 fold and 4.8 fold increase in caspase-9 and caspase-3 activity, respectively, occurs. the measurement of mitochondrial membrane potential of a cationic dye (JC-1) showed a decrease in mitochondrial membrane potential in both HeLa and MCF-7 cells depicting that compound might have adopted intrinsic pathway of apoptosis. Ability of Mn(phen)(2)Cl-2 to interact with HS-DNA demonstrates hyperchromicity with slight blue shift from 269 nm to 265 nm showing a non-covalent interaction with Gibbs free energy of Delta G = -14.62 kJ/mol. Communicated by Ramaswamy H. Sarma

Kaynak

Journal of Biomolecular Structure & Dynamics

Cilt

39

Sayı

3

Bağlantı

https://doi.org/10.1080/07391102.2020.1726818
https://hdl.handle.net/11436/1191

Koleksiyonlar

  • FEF, Kimya Bölümü Koleksiyonu [474]
  • PubMed İndeksli Yayınlar Koleksiyonu [2443]
  • Scopus İndeksli Yayınlar Koleksiyonu [5931]
  • WoS İndeksli Yayınlar Koleksiyonu [5260]



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